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Blood and aqueous humor samples acquired from 50 patients with POAG and 50 control subjects were used for QPCR and protein levels analysis by ELISA.In vivo promoter activity assays were carried on HTM cells using dual luciferase assay.The samples were immediately (after mixing with protein inhibitors cocktail) frozen in liquid nitrogen and stored at 80°C.Medical history was obtained from all subjects, and no one reported present or former cancer or any genetic disease.Women comprised 55% of OAG, 70% of ACG, and 59% of all glaucoma in 2010.Bilateral blindness were present in 4.5 million people with OAG and 3.9 million people with ACG in 2010, rising to 5.9 and 5.3 million people in 2020, respectively [1, 2].Thus, the aim of this study was to evaluate expression level of MMP1, MMP9, MMP12, and TIMP1 in blood and aqueous humor of POAG patients compared to control group of people without any type of glaucoma diagnosed.Moreover, we compared expression levels with polymorphic variants previously determined by our team [12].

The elevated intraocular pressure (IOP) is considered to be the main risk factor in biomechanical theory of POAG development [3].

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The aim of presented work was to analyze the impact of particular polymorphic changes in the promoter regions of the -1607 1G/2G MMP1, -1562 C/T MMP9, -82 A/G MMP12, -511 C/T IL-1β, and 372 T/C TIMP1 genes on their expression level in POAG patients.

Cells were isolated from juxtacanalicular and corneoscleral region of human eye.

Culture flasks were coated with poly-L-lysine (2 μg/cm).

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